|
KMID : 0617220000110010011
|
|
Duksung Bulletin Phamaceutical Sciences
2000 Volume.11 No. 1 p.11 ~ p.16
|
|
|
Induction of Apoptosis by Capsaicin in Hepatocarcinoma Cells
|
|
|
|
|
Abstract
|
|
|
|
Hepatocellular carcinoma is one of the most lethal malignancies and there is no effective preventive measure in this highly malignant disease to date. In the present study, we investigated the chemopreventive potential of capsaicin (8-methyl-N-vanillyl-6-nonenamide), the principal pungent ingredient found in hot red pepper, in SK-Hep-1 hepatocellular carcinoma cells. Treatment of capsaicin inhibited growth of SK-Hep-1 cells in a concentration-dependent manner. The inhibitory effect of capsaicin on SK-Hep-1 cell growth was mainly due to the induction of apoptosis as evidenced by DNA fragmentation and nuclear condensation. Furthermore, capsaicin prominently reduced the ratio of anti-apoptotic Bcl-2 to proapoptotic Bax. We also show that the activity of DEVDase (caspase-3 like) is more likely involved than that of YVADase (caspase-1 like) in capsaicin-induced growth inhibition. These results deminstrate that capsaicin efficiently induced apoptosis in SK-Hep-1 cells through a caspase-3-dependent mechanism, which may contribute to the chemopreventive function for hepatocellular carcinoma.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|